The D-type alfalfa cyclin gene cycMs4 complements G1 cyclin-deficient yeast and is induced in the G1 phase of the cell cycle

Dahl, M., Meskiene, I., Bogre, L., Ha, D. T., Swoboda, I., Hubmann, R., Hirt, H. and Heberle-Bors, E.

(1995)

Dahl, M., Meskiene, I., Bogre, L., Ha, D. T., Swoboda, I., Hubmann, R., Hirt, H. and Heberle-Bors, E. (1995) The D-type alfalfa cyclin gene cycMs4 complements G1 cyclin-deficient yeast and is induced in the G1 phase of the cell cycle. Plant Cell, 7 (11).

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Abstract

Cyclins are key regulators of the cell cycle in all eukaryotes. In alfalfa, we have previously isolated three B-type cyclins. The closely related cycMs1 and cycMs2 genes are expressed primarily during the G2 and M phases and are most likely mitotic cyclins; expression of the cycMs3 gene is induced in the G0-to-G1 transition, when cells reenter the cell cycle. By complementation of G1 cyclin-deficient yeast cells, a novel alfalfa cyclin, designated cycMs4, was isolated. The predicted amino acid sequence of the cycMs4 gene is most similar to that of the Arabidopsis cyclin delta 3 gene. CycMs4 and cyclin delta 3 belong to the class of D-type cyclins and contain PEST-rich regions and a retinoblastoma binding motif. When comparing expression levels in different organs, cycMs4 transcripts were present predominantly in roots. Whereas expression of the cycMs4 gene was cell cycle-regulated in suspension-cultured cells, transcription in roots was observed to depend also on the positional context of the cell. When differentiated G0-arrested leaf cells were induced to resume cell division by treatment with plant hormones, cycMs4 transcription was induced before the onset of DNA synthesis. Whereas this induction was preceded by that of the cycMs3 gene, cycMs2 expression occurred later and at the same time as mitotic activity. These data suggest that cycMs4 plays a role in the G1-to-S transition and provide a model to investigate the plant cell cycle at the molecular level.

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This is a Submitted version
This version's date is: 1995
This item is not peer reviewed

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https://repository.royalholloway.ac.uk/items/d005b3d6-c916-46b0-46bc-39b8dc998f01/3/

Item TypeJournal Article
TitleThe D-type alfalfa cyclin gene cycMs4 complements G1 cyclin-deficient yeast and is induced in the G1 phase of the cell cycle
AuthorsDahl, M.
Meskiene, I.
Bogre, L.
Ha, D. T.
Swoboda, I.
Hubmann, R.
Hirt, H.
Heberle-Bors, E.
Uncontrolled KeywordsAmino Acid Sequence Base Sequence Cell Cycle/*physiology Cyclins/*biosynthesis/genetics G1 Phase *Gene Expression *Genes, Plant Genetic Complementation Test Medicago sativa/cytology/*genetics/metabolism Meristem Molecular Sequence Data *Plant Proteins RNA, Plant/isolation & purification/metabolism Recombinant Proteins/biosynthesis Saccharomyces cerevisiae/cytology/genetics/growth & development
DepartmentsFaculty of Science\Biological Science

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Deposited by Research Information System (atira) on 27-Jan-2013 in Royal Holloway Research Online.Last modified on 27-Jan-2013

Notes

1040-4651 (Print) 1040-4651 (Linking) Comparative Study Journal Article Research Support, Non-U.S. Gov't


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