George Dickson, Timoleon Papagatsias, George Rozis, Takis Athanasopoulos, Frances Gotch and Steven Patterson (2008) Activity of different vaccine-associated promoter elements in human dendritic cells. Immunology Letters, 115 (2). pp. 117-125 . ISSN 0165-2478
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Vaccine design approaches that target dendritic cells (DC) aim at achieving high levels of transgene expression. Careful selection of the promoter element driving the foreign gene is therefore important. We have constructed adenovirus vectors carrying the gene for enhanced green fluorescent protein (eGFP) driven by three different promoters, CMV, CMV5 and Ubiquitin C (UbC) promoter, and analysed their activity in different populations of human DC, namely blood plasmacytoid (pDC) and myeloid DC (mDC), monocyte-derived DC (moDC), Langerhans (LC) and dermal type DC (dDC). Although the CMV5 promoter was more active than the other two promoters in the HeLa and 911HER cell lines, in human DC the highest level of transgene expression was seen with the CMV promoter. There was very low level eGFP expression in all cell types transduced with the UbC promoter. Highest eGFP expression levels were observed in moDC, cultured mDC and LC and the lowest levels in pDC. Expression of eGFP was augmented in all DC populations upon stimulation with CD40 ligand (CD40L). These findings demonstrate that the CMV promoter is the most effective of the three promoters tested in a range of different human DC populations.
This is a Draft version This version's date is: 29/01/2008 This item is peer reviewed
https://repository.royalholloway.ac.uk/items/3b5af064-4400-a1df-cd13-653c539293f8/1/
Deposited by () on 31-Mar-2010 in Royal Holloway Research Online.Last modified on 15-Dec-2010
(C) 2007 Elsevier, whose permission to mount this version for private study and research is acknowledged. The repository version is the author's final draft.
[1] Banchereau J, Steinman RM. Dendritic cells and the control of immunity. Nature 1998; 392(6673):245-252.
[2] Kaisho T, Akira S. Regulation of dendritic cell function through toll-like receptors. Curr Mol Med 2003; 3(8):759-771.
[3] Shortman K, Liu YJ. Mouse and human dendritic cell subtypes. Nature Reviews Immunology 2002; 2(3):151-161.
[4] Demedts IK, Bracke KR, Maes T, Joos GF, Brusselle GG. Different roles for human lung dendritic cell subsets in pulmonary immune defense mechanisms. Am J Respir Cell Mol Biol 2006; 35(3):387-393.
[5] Lappin MB, Kimber I, Norval M. The role of dendritic cells in cutaneous immunity. Arch Dermatol Res 1996; 288(3):109-121.
[6] Nestle FO, Filgueira L, Nickoloff BJ, Burg G. Human dermal dendritic cells process and present soluble protein antigens. J Invest Dermatol 1998; 110(5):762-766.
[7] Caux C, Vanbervliet B, Massacrier C, DezutterDambuyant C, deSaintVis B, Jacquet C et al. CD34+ hematopoietic progenitors from human cord blood differentiate along two independent dendritic cell pathways in response to GM-CSF+TNF alpha. J Exp Med 1996; 184(2):695-706.
[8] Ratzinger G, Baggers J, de Cos MA, Yuan JD, Dao T, Reagan JL et al. Mature human Langerhans cells derived from CD34+ hematopoietic progenitors stimulate greater cytolytic T lymphocyte activity in the absence of bioactive IL-12p70, by either single peptide presentation or cross-priming, than do dermal-interstitial or monocyte-derived dendritic cells. J Immunol 2004; 173(4):2780-2791.
[9] Romani N, Kampgen E, Koch F, Heufler C, Schuler G. Dendritic cell production of cytokines and responses to cytokines. Int Rev Immunol 1990; 6(2-3):151-161.
[10] Sallusto F, Lanzavecchia A. Efficient presentation of soluble-antigen by cultured human dendritic cells is maintained by granulocyte-macrophage colony-stimulating factor plus interleukin-4 and down-regulated by tumor-necrosis-factor-alpha. J Exp Med 1994; 179(4):1109-1118.
[11] Nenoi M, Cartwright IL, Mita K, Ichimura S. Comparison of the 5' upstream region of the evolutionarily equivalent polyubiquitin gene of humans and Chinese hamsters. Gene 1996; 179(2):297-299.
[12] Schorpp M, Jager R, Schellander K, Schenkel J, Wagner EF, Weiher H et al. The human ubiquitin C promoter directs high ubiquitous expression of transgenes in mice. Nucleic Acids Res 1996; 24(9):1787-1788.
[13] Massie B, Couture F, Lamoureux L, Mosser DD, Guilbault C, Jolicoeur P et al. Inducible overexpression of a toxic protein by an adenovirus vector with a tetracycline-regulatable expression cassette. J Virol 1998; 72(3):2289-2296.
[14] Massie B, Mosser DD, Koutroumanis M, Vitte-Mony I, Lamoureux L, Couture F et al. New adenovirus vectors for protein production and gene transfer. Cytotechnology 1998; 28(1-3):53-64.
[15] Mosser DD, Caron AW, Bourget L, Jolicoeur P, Massie B. Use of a dicistronic expression cassette encoding the green fluorescent protein for the screening and selection of cells expressing inducible gene products. Biotechniques 1997; 22(1):150-161.
[16] Hall TA. BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp Ser 1999; 41:95-98.
[17] Quandt K, Frech K, Karas H, Wingender E, Werner T. MatInd and MatInspector: New fast and versatile tools for detection of consensus matches in nucleotide sequence data. Nucleic Acids Res 1995; 23(23):4878-4884.
[18] He TC, Zhou SB, da Costa LT, Yu J, Kinzler KW, Vogelstein B. A simplified system for generating recombinant adenoviruses. Proc Natl Acad Sci U S A 1998; 95(5):2509-2514.
[19] Zeng M, Smith SK, Siegel F, Shi Z, Van Kampen KR, Elmets CA et al. AdEasy system made easier by selecting the viral backbone plasmid preceding homologous recombination. Biotechniques 2001; 31(2):260-262.
[20] Freundlich B, Avdalovic N. Use of gelatin plasma coated flasks for isolating human peripheral-blood monocytes. J Immunol Methods 1983; 62(1):31-37.
[21] Sato T, Terai M, Yasuda R, Watanabe R, Berd D, Mastrangelo MJ et al. Combination of monocyte-derived dendritic cells and activated T cells which express CD40 ligand: a new approach to cancer immunotherapy. Cancer Immunol Immunother 2004; 53(1):53-61.
[22] Graf D, Korthauer U, Mages HW, Senger G, Kroczek RA. Cloning of Trap, a ligand for CD40 on human T-cells. Eur J Immunol 1992; 22(12):3191-3194.
[23] Nenoi M, Mita K, Ichimura S, Cartwright IL, Takahashi EI, Yamauchi M et al. Heterogeneous structure of the polyubiquitin gene UbC of HeLa S3 cells. Gene 1996; 175(1-2):179-185.
[25] Mondesert G, Tribouley C, Kedinger C. Identification of a novel downstream binding-protein implicated in late-phase-specific activation of the adenovirus major late promoter. Nucleic Acids Res 1992; 20(15):3881-3889.
[26] Parks CL, Shenk T. Activation of the adenovirus major late promoter by transcription factors MAZ and Sp1. J Virol 1997; 71(12):9600-9607.
[27] Iacobelli-Martinez M, Nemerow GR. Preferential activation of Toll-like receptor nine by CD46-utilizing adenoviruses. J Virol 2007; 81(3):1305-12.
[28] Lore K, Adams WC, Havenga MJ, Precopio ML, Holterman L, Goudsmit J et al. Myeloid and plasmacytoid dendritic cells are susceptible to recombinant adenovirus vectors and stimulate polyfunctional memory T cell responses. J Immunol 2007; 179(3):1721-9.
[29] Bontkes HJ, Ruizendaal JJ, Schreurs MWJ, Kramer D, Meijer CJLM, Hooijberg E. Antigen gene transfer to human plasmacytoid dendritic cells using recombinant adenovirus and vaccinia virus vectors. Cell Oncol 2005; 27(3):175-82.
[30] Huarte E, Larrea E, Hernández-Alcoceba R, Alfaro C, Murillo O, Arina A et al. Recombinant adenoviral vectors turn on the type I interferon system without inhibition of transgene expression and viral replication. Mol Ther 2006; 14(1):129-38.
[31] Penuelas I, Mazzolini G, Boan JF, Sangro B, Marti-Climent J, Ruiz M et al. Positron emission tomography imaging of adenoviral-mediated transgene expression in liver cancer patients. Gastroenterology 2005; 128(7):1787-95.
[32] Hummel M, Abecassis MM. A model for reactivation of CMV from latency. J Clin Virol 2002; 25:S123-S136.
[33] Marinovic AC, Zheng B, Mitch WE, Price SR. Ubiquitin (UbC) expression in muscle cells is increased by glucocorticoids through a mechanism involving Sp1 and MEK1. J Biol Chem 2002; 277(19):16673-16681.
[34] Perkins ND, Edwards NL, Duckett CS, Agranoff AB, Schmid RM, Nabel GJ. A cooperative interaction between Nf-kappa-B and Sp1 is required for HIV-1 enhancer activation. EMBO J 1993; 12(9):3551-3558.
[35] Rea D, Schagen FH, Hoeben RC, Mehtali M, Havenga MJ, Toes RE et al. Adenoviruses activate human dendritic cells without polarization toward a T-helper type 1-inducing subset. J Virol. 1999; 73(12):10245-53.
[36] Rozis G, de Silva S, Benlahrech A, Papagatsias T, Harris J, Gotch F et al. Langerhans cells are more efficiently transduced than dermal dendritic cells by adenovirus vectors expressing either group C or group B fibre protein: Implications for mucosal vaccines. Eur J Immunol 2005; 35(9):2617-2626.