Gamma-tubulin is essential for acentrosomal microtubule nucleation and coordination of late mitotic events in Arabidopsis

Binarova, P., Cenklova, V., Prochazkova, J., Doskocilova, A., Volc, J., Vrlik, M. and Bogre, L.

(2006)

Binarova, P., Cenklova, V., Prochazkova, J., Doskocilova, A., Volc, J., Vrlik, M. and Bogre, L. (2006) Gamma-tubulin is essential for acentrosomal microtubule nucleation and coordination of late mitotic events in Arabidopsis. Plant Cell, 18 (5).

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Abstract

Gamma-tubulin is required for microtubule (MT) nucleation at MT organizing centers such as centrosomes or spindle pole bodies, but little is known about its noncentrosomal functions. We conditionally downregulated gamma-tubulin by inducible expression of RNA interference (RNAi) constructs in Arabidopsis thaliana. Almost complete RNAi depletion of gamma-tubulin led to the absence of MTs and was lethal at the cotyledon stage. After induction of RNAi expression, gamma-tubulin was gradually depleted from both cytoplasmic and microsomal fractions. In RNAi plants with partial loss of gamma-tubulin, MT recovery after drug-induced depolymerization was impaired. Similarly, immunodepletion of gamma-tubulin from Arabidopsis extracts severely compromised in vitro polymerization of MTs. Reduction of gamma-tubulin protein levels led to randomization and bundling of cortical MTs. This finding indicates that MT-bound gamma-tubulin is part of a cortical template guiding the microtubular network and is essential for MT nucleation. Furthermore, we found that cells with decreased levels of gamma-tubulin could progress through mitosis, but cytokinesis was strongly affected. Stepwise diminution of gamma-tubulin allowed us to reveal roles for MT nucleation in plant development, such as organization of cell files, anisotropic and polar tip growth, and stomatal patterning. Some of these functions of gamma-tubulin might be independent of MT nucleation.

Information about this Version

This is a Submitted version
This version's date is: 2006
This item is not peer reviewed

Link to this Version

https://repository.royalholloway.ac.uk/items/2447177a-b423-481f-1d8e-a48dc6de32ea/6/

Item TypeJournal Article
TitleGamma-tubulin is essential for acentrosomal microtubule nucleation and coordination of late mitotic events in Arabidopsis
AuthorsBinarova, P.
Cenklova, V.
Prochazkova, J.
Doskocilova, A.
Volc, J.
Vrlik, M.
Bogre, L.
Uncontrolled KeywordsArabidopsis/anatomy & histology/*cytology/metabolism Arabidopsis Proteins/genetics/*physiology Cell Enlargement Cell Nucleus/physiology Down-Regulation Microtubules/metabolism/*ultrastructure Mitosis/*physiology Molecular Sequence Data Phenotype Plant Leaves/cytology/growth & development/metabolism Plant Roots/cytology/growth & development/metabolism RNA Interference Tubulin/genetics/*physiology
DepartmentsFaculty of Science\Biological Science

Identifiers

doihttp://dx.doi.org/10.1105/tpc.105.038364

Deposited by Research Information System (atira) on 18-Nov-2014 in Royal Holloway Research Online.Last modified on 18-Nov-2014

Notes

1040-4651 (Print) 1040-4651 (Linking) Journal Article Research Support, Non-U.S. Gov't


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